Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: HSPA6 is induced by RIG-I-like receptors and negatively regulates type-I interferon signaling

doi: 10.1007/s00018-026-06147-8

Figure Lengend Snippet: HSPA6 is upregulated in expression by IFN signaling-competent MDA5. A . Volcano plot of RNA-seq data comparing gene expression between WT and GOF MDA5 expressed HEK293 cells. B . HEK293T cells were transfected with plasmids expressing WT or GOF MDA5. 24 h later, cells were lysed for RNA detection by RT-qPCR and protein detection by western blotting. EV, empty vector. C . HEK293T cells were mock-treated or treated with recombinant IFNβ; at a final concentration of 10 ng/ml for 24 h. RNA was purified, and gene expression was tested by RT-qPCR. D . HEK293T cells were transfected with plasmids expressing MDA5. 24 h later, cells were lysed for RNA detection. The qPCR primer-targeting regions on AL590385.23 was indicated in green. E . HEK293T cells were transfected with MDA5 expressing plasmid. 6 h post transfection, cells were transfected with poly I: C at a final concentration of 0.8 ng/μ;l. 18 h later, cells were lysed for RNA measurement. F . Cells were transfected with GOF MDA5 expressing plasmid and harvested at indicated hours post transfection. RNA was measured by RT-qPCR. G , H . Act.D was added at a final concentration of 5 μ;g/ml 24 h post transfection of plasmids indicated. 24 h post treatment, cells were lysed for RNA measurement. I , J . HEK293T cells were infected with EMCV at an MOI of 0.01. 24 h post infection, cells were lysed for RNA measurement. K . RNA levels of IFNβ; and HSPA6 in epithelial cells were shown upon SARS-CoV infection. The data was obtained from GEO database (accession number: GSE17400 ). For B-E, G-J, data are means ± SD of three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001. For F, data are means ± SD of two independent experiments

Article Snippet: The recombinant human IFNβ (Sino Biological, 10704-HNAS) was dissolved in PBS at a stock concentration of 1.0 mg/ml as the manufacture’s instruction suggested.

Techniques: Expressing, RNA Sequencing, Gene Expression, Transfection, RNA Detection, Quantitative RT-PCR, Western Blot, Plasmid Preparation, Recombinant, Concentration Assay, Purification, Infection

Journal: Materials Today Bio

Article Title: Multiple-pathway cGAS-STING activation with enhanced mild photothermal therapy through glycolysis regulation for boosting gastric cancer immunotherapy

doi: 10.1016/j.mtbio.2026.102790

Figure Lengend Snippet: In vitro antitumor efficacy of the nanoparticles, ICD induction, and activation of the cGAS-STING pathway. Uptake of MOMn and MOMn@MB by MFC cells at (a) 4 and (b) 6 h. (c) Calcein-AM/PI and (d) JC-1 staining fluorescence images of MFC cells after treatment with different groups. Cytotoxicity analysis of MFC cells treated with (e) MO@MB and (f) MOMn@MB. Western blot analysis of (g) glycolysis pathway related proteins and (h) HSP70/90 in MFC cells treated with different nanoparticles. (i) CLSM images of CRT exposure on the surface of MFC cells after different treatments. (j) DNA damage assessment via γ-H2AX immunostaining in MFC cells treated with different nanoparticles. Levels of (k) lactate, (l) ATP, (m) HMGB1, and (n) IFN-β in MFC cells treated with different nanoparticles. (o) Western blot analysis of cGAS-STING pathway related proteins with different treatment. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. G1-G6 are denoted as Control, NIR Laser, MO@MB, MO@MB + NIR Laser, MOMn@MB, and MOMn@MB + NIR Laser groups, respectively.

Article Snippet: The mouse HMGB1 enzyme-linked immunosorbent assay (ELISA) kit and mouse interferon-β (IFN-β) ELISA kit were purchased from Wuhan Elabscience Biotechnology Co., Ltd.

Techniques: In Vitro, Activation Assay, Staining, Fluorescence, Western Blot, Immunostaining, Control

Journal: Materials Today Bio

Article Title: Multiple-pathway cGAS-STING activation with enhanced mild photothermal therapy through glycolysis regulation for boosting gastric cancer immunotherapy

doi: 10.1016/j.mtbio.2026.102790

Figure Lengend Snippet: DC maturation and activation of the cGAS-STING pathway. (a) Schematic diagram of the extraction from BMDCs to DC maturation and the Transwell assay. Created by Biorender. (b) Expression of CD80 and CD86 quantitatively determined by flow cytometry analysis with different treatments, and (c) the average DC maturation rate based on the results. (d) The levels of IFN-β in DC cells with different treatments. (e) Western blot analysis was employed to evaluate the expression of cGAS-STING pathway-related proteins in DC cells across different treatments. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. G1-G6 are denoted as Control, NIR Laser, MO@MB, MO@MB + NIR Laser, MOMn@MB, and MOMn@MB + NIR Laser groups, respectively.

Article Snippet: The mouse HMGB1 enzyme-linked immunosorbent assay (ELISA) kit and mouse interferon-β (IFN-β) ELISA kit were purchased from Wuhan Elabscience Biotechnology Co., Ltd.

Techniques: Activation Assay, Extraction, Transwell Assay, Expressing, Flow Cytometry, Western Blot, Control

Journal: Inflammation

Article Title: IRF7 Modulates Inflammatory Pain Through Upregulating IFNβ in Mice Trigeminal Ganglion

doi: 10.1007/s10753-026-02483-w

Figure Lengend Snippet: Expression of IRF7 and IFNβ in the TG during CFA-induced inflammatory pain. ( A ) Quantification of IFNβ levels in the TG and serum by ELISA before and after injection of CFA at 6 h, 1 d, 3 d post-CFA injection.( B ) Quantitative analysis of mRNA expression of Ifnb and Irf7 in the TG at 6 h, 1, 3 and 7 d post-CFA injection. ( C ) Time course of IRF7 and IFNβ expression in the TG during orofacial inflammatory pain and quantitative analysis of their expression levels normalized to GAPDH. ( D ) Quantitative analysis of mRNA expression of the key upstream factors (Sting1, Tlr4, Tlr3 and Irf3) related to IFNβ synthesis. Data are expressed as mean ± SD; N= 3-5 per group; one-way ANOVA followed by post hoc test was performed to assess significance between groups (*P < 0.05, **P < 0.01)

Article Snippet: IFNβ levels in serum and TG were quantified using ELISA kits (E-EL-M0033, Elabscience Biotechnology), according to the manufacturer’s instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Injection